Fig. 2: DNAJC5 enhances proliferation and migration of LUAD cells.

A Protein levels of DNAJC5 were analyzed in four different LUAD cell lines using Western blot analysis. B Western blot analysis was performed to assess the protein levels of DNAJC5 in the stable DNAJC5-overexpressing A549 cell line. C Cell viability assays using CCK8 showed increased proliferation as indicated (right). D Colony formation assays demonstrated enhanced colony formation, as shown. E Trans-well assays were conducted to evaluate cell migration rates (left), and the results were quantified (right). F–I Knockdown of DNAJC5 suppressed H1299 cell proliferation. F Interference efficiency of DNAJC5 was confirmed by Western blot analysis. G Cell viability assays using CCK8 revealed decreased proliferation as indicated. H The results of colony formation assay demonstrated reduced colony formation. I Trans-well assays were performed to assess cell migration rates (left), and the results were quantified (right).