Focus stacking GUI for microscopy/macro stacks (developed for the OpenScan community).

uv sync
uv run photostacker

1. Click Load Images.
2. Keep Stack Detection = Auto unless your naming needs Fixed size or Regex.
3. Optionally enable Auto Tune.
4. Choose:
   • Focus Measure
   • Blending Method
5. Click Process Stack.

Settings persist to %APPDATA%/photo_focus_stacker_settings.json (Windows).

• Alignment Pyramid Levels: 4-5
• Alignment Mask Threshold: 8-12
• Focus Window Size: 5-7
• Focus Measure: Tenengrad (good in low-contrast / flat regions)
• Blend: Guided Weighted (Edge-Aware) or Luma Weighted + Chroma Pick (MFF)
• Sharpen: 0.0 (sharpen later if needed)

• Alignment Pyramid Levels: 1-2
• Focus Window Size: 7
• Focus Measure: Laplacian Variance
• Blend: Direct Map Selection (fastest, can artifact)

• Increase Focus Window Size (e.g. 9)
• Prefer Guided Weighted or MFF over Direct Map
• Reduce Sharpen (keep 0.0 while tuning)

Controls Pyramid ECC alignment from coarse-to-fine.

• Higher (4-6)
  • More robust to larger shifts/rotations.
  • Slower.
• Lower (1-2)
  • Faster.
  • Can fail if the stack has motion.

Controls how many pixels are used by ECC by building a gradient mask from the reference image.

• Lower value (stricter)
  • Uses only the strongest edges.
  • More robust to noise, but can fail if the subject is low-texture.
• Higher value (more permissive)
  • Includes more pixels (weaker edges).
  • Can help low-contrast scenes, but can be influenced by noise.

Size of the local window used to aggregate the focus measure (odd numbers only).

• Smaller (3-5)
  • Sharper transitions, more detail.
  • More sensitive to noise.
• Larger (7-11)
  • Smoother maps (less speckle).
  • Can slightly soften fine focus boundaries.

• Laplacian Variance (laplacian_var)
  • Very common and fast.
  • Can underperform in flat/homogeneous regions.
• Tenengrad (tenengrad)
  • Uses gradient energy.
  • Often better in low-contrast regions.
• SML (sml)
  • Sum-modified Laplacian (|Lx|+|Ly|).
  • Often a good alternative to Laplacian variance on textured subjects.

Unsharp mask applied after blending.

• 0.0: recommended while tuning
• 0.3-0.8: mild sharpening
• > 1.0: can create halos/noise

• Weighted Blending
  • Smooth, stable baseline.
• Guided Weighted (Edge-Aware)
  • Smooths weights in an edge-aware way (guided filter when available).
  • Good general-purpose “quality” option.
• Direct Map Selection
  • Chooses sharpest frame per pixel (plus top-2 confidence blending in ambiguous areas).
  • Can produce artifacts if focus maps are noisy.
• Laplacian Pyramid Fusion
  • Multi-scale fusion; helps preserve detail at multiple scales.
• Luma Weighted + Chroma Pick (MFF)
  • Fuses luminance smoothly and selects/blends chroma from the sharpest frames.
  • Often reduces color seams/halos.

• General quality

  • Levels 4
  • Mask 8-12
  • Window 5-7
  • Focus Tenengrad
  • Blend Guided Weighted or MFF
  • Sharpen 0.0

• Low-texture subjects

  • Focus Tenengrad
  • Mask threshold slightly higher (more permissive)
  • Blend Guided Weighted or MFF

• Direct Map + small window (3–5)

  • Noisy focus maps turn into pixel-level artifacts.

• High sharpening + any artifacts

  • Sharpening amplifies halos/noise. Tune sharpening last.

• Very low alignment levels (1) with motion

  • Misalignment creates ghosting/blur no blend can fix.

• Laplacian Variance: local variance of Laplacian in a window.
• Tenengrad: local average of gradient energy (Gx^2 + Gy^2).
• SML: sum-modified Laplacian |Lx| + |Ly| averaged in a window.

For speed, focus maps are computed on a downscaled grayscale image for large inputs and upsampled back.

• Weighted Blending: soft weights from focus maps.
• Guided Weighted (Edge-Aware): weight maps are edge-aware smoothed (guided/bilateral) before blending.
• Direct Map Selection: picks the sharpest source per pixel, with a top-2 confidence blend in ambiguous regions.
• Laplacian Pyramid Fusion: multi-scale fusion using Laplacian pyramids.
• Luma Weighted + Chroma Pick (MFF): fuse luminance smoothly and select/blend chroma from the sharpest frames (reduces color seams).

Alignment (ECC) is the slowest stage. The stacker caches aligned_images + focus_maps (bounded) so rerunning the same stack with the same alignment/focus settings can reuse intermediates.

For each stack:

1. Load RGB images as float32 [0,1].

2. Align each frame to the first frame using pyramid ECC.

   We estimate a transform by maximizing the Enhanced Correlation Coefficient (ECC) objective between the reference and moving image (at multiple resolutions). A gradient-based mask is used to emphasize informative pixels.

   • Primary model: homography H (3x3)
   • Fallback model: affine (2x3) if homography ECC fails

3. Compute a focus map per aligned frame.

   Each focus measure returns a non-negative field f_i(x):

   • Laplacian variance: Var(ΔI) in a local window
   • Tenengrad: local mean of Gx^2 + Gy^2
   • SML: local mean of |Lx| + |Ly|

4. Convert focus maps to weights.

   For weight-based blends we use a stable per-pixel softmax:

   w_i(x) = exp(beta * f_i(x)) / sum_j exp(beta * f_j(x))

   This avoids hard seams and keeps weights normalized.

5. Blend using the selected method.

   • Weighted / Guided Weighted: I(x) = sum_i w_i(x) * I_i(x)
   • Direct Map: choose argmax focus index (with top-2 confidence mixing in ambiguous areas)
   • Laplacian Pyramid: multi-scale fusion of Laplacian pyramids with Gaussian pyramids of weights
   • MFF (luma/chroma): fuse luminance with weights and pick/blend chroma from sharpest frames

6. Optional unsharp mask sharpening.

Python 3.9+.

git clone https://github.com/sha5b/Photo-Focus-Stacker.git
cd Photo-Focus-Stacker
uv sync
uv run photostacker

from src.core.focus_stacker import FocusStacker

stacker = FocusStacker(
    num_pyramid_levels=4,
    gradient_threshold=10,
    focus_window_size=7,
    focus_measure_method="tenengrad",
    blend_method="guided_weighted",
    sharpen_strength=0.0,
)

result = stacker.process_stack(["img0.jpg", "img1.jpg", "img2.jpg"], color_space="sRGB")

• num_pyramid_levels: 1–6
• gradient_threshold: 1–100
• focus_window_size: 3–21 (odd)
• focus_measure_method: "laplacian_var" | "tenengrad" | "sml"
• blend_method:
  • "weighted"
  • "guided_weighted"
  • "direct_map"
  • "laplacian_pyramid"
  • "luma_weighted_chroma_pick"
• sharpen_strength: 0.0–3.0

Non-Commercial Open Source License (NCOSL). See LICENSE.