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Title:
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Authors:
Boch, Jens; Scholze, Heidi; Schornack, Sebastian; Landgraf, Angelika; Hahn, Simone; Kay, Sabine; Lahaye, Thomas; Nickstadt, Anja; Bonas, Ulla
Publication:
Science, Volume 326, Issue 5959, pp. 1509- (2009). (Sci Homepage)
Publication Date:
12/2009
Category:
BOTANY
Origin:
SCIENCE
Abstract Copyright:
(c) 2009: Science
DOI:
10.1126/science.1178811
Bibliographic Code:
2009Sci...326.1509B

Abstract

The pathogenicity of many bacteria depends on the injection of effector proteins via type III secretion into eukaryotic cells in order to manipulate cellular processes. TAL (transcription activator-like) effectors from plant pathogenic Xanthomonas are important virulence factors that act as transcriptional activators in the plant cell nucleus, where they directly bind to DNA via a central domain of tandem repeats. Here, we show how target DNA specificity of TAL effectors is encoded. Two hypervariable amino acid residues in each repeat recognize one base pair in the target DNA. Recognition sequences of TAL effectors were predicted and experimentally confirmed. The modular protein architecture enabled the construction of artificial effectors with new specificities. Our study describes the functionality of a distinct type of DNA binding domain and allows the design of DNA binding domains for biotechnology.
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