PIPES
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This article is about the biochemical buffer PIPES. For other uses, see Pipe (disambiguation).
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| Names | |
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| IUPAC name
1,4-Piperazinediethanesulfonic acid (IUPAC)
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| Other names
PIPES
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| Identifiers | |
5625-37-6  |
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| ChemSpider | 72022  |
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| Jmol interactive 3D | Image |
| PubChem | 6992709 |
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| Properties | |
| C8H18N2O6S2 | |
| Molar mass | 302.37 |
| Appearance | White powder |
| Melting point | Decomposes above 300 °C |
| Boiling point | Decomposes |
| 1 g/L (100 °C) | |
| Hazards | |
| Main hazards | Irritant |
| Safety data sheet | External MSDS |
| NFPA 704 | |
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Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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| Infobox references | |
PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), and frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]
Applications[edit]
PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25° C. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.
See also[edit]
References[edit]
- ^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
- ^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
- ^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
- ^ Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095.
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