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Title:
Essential genes of a minimal bacterium
Authors:
Glass, John I.; Assad-Garcia, Nacyra; Alperovich, Nina; Yooseph, Shibu; Lewis, Matthew R.; Maruf, Mahir; Hutchison, Clyde A., III; Smith, Hamilton O.; Venter, J. Craig
Publication:
Proceedings of the National Academy of Science, vol. 103, Issue 2, p.425-430
Publication Date:
01/2006
Category:
MICROBIOLOGY
Origin:
PNAS
DOI:
10.1073/pnas.0510013103
Bibliographic Code:
2006PNAS..103..425G

Abstract

Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport. Genes encoding proteins of unknown function constitute 28% of the essential protein-coding genes set. Disruption of some genes accelerated M. genitalium growth. minimal cell | Mycoplasma genitalium | synthetic biology | transposon mutagenesis
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