FHL2

Four and a half LIM domains 2
	; PDB rendering based on 1x4k.
Available structures
PDB	Ortholog search: PDBe, RCSB
List of PDB id codes
	1X4K, 1X4L, 2D8Z
Identifiers
Symbols	FHL2; AAG11; DRAL; FHL-2; SLIM-3; SLIM3
External IDs	OMIM: 602633 MGI: 1338762 HomoloGene: 20372 GeneCards: FHL2 Gene
Gene Ontology
Molecular function	• transcription coactivator activity; • protein binding; • transcription factor binding; • zinc ion binding; • identical protein binding; • metal ion binding; • androgen receptor binding;
Cellular component	• nucleus; • nucleoplasm; • focal adhesion; • actin cytoskeleton; • Z disc; • M band;
Biological process	• negative regulation of transcription from RNA polymerase II promoter; • osteoblast differentiation; • transcription, DNA-dependent; • response to hormone stimulus; • androgen receptor signaling pathway; • negative regulation of apoptotic process; • cellular lipid metabolic process; • small molecule metabolic process; • positive regulation of transcription, DNA-dependent; • atrial cardiac muscle cell development; • ventricular cardiac muscle cell development; • heart trabecula formation;
	Sources: Amigo / QuickGO
RNA expression pattern
	More reference expression data
Orthologs
	This box: view; talk; edit;

Four and a half LIM domains protein 2 is a protein that in humans is encoded by the FHL2 gene.^[1]

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]^[2]

[edit] Interactions

FHL2 has been shown to interact with Beta-catenin,^[3] Titin,^[4] Zinc finger and BTB domain-containing protein 16,^[5] Integrin, beta 6,^[6] Androgen receptor,^[7] ITGA7,^[8] CREB1,^[9] MAPK1,^[10] CD49c,^[6] ZNF638,^[11] BRCA1,^[12]^[13] FHL3,^[9]^[14] IGFBP5,^[15] TRAF6,^[16] EIF6,^[6] CD18,^[6] CD29^[6] and PSEN2.^[17]

[edit] References

^ Morgan MJ, Madgwick AJ (October 1996). "Slim defines a novel family of LIM-proteins expressed in skeletal muscle". Biochem. Biophys. Res. Commun. 225 (2): 632–8. doi:10.1006/bbrc.1996.1222. PMID 8753811.
^ "Entrez Gene: FHL2 four and a half LIM domains 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=2274.
^ Wei, Yu; Renard Claire-Angélique, Labalette Charlotte, Wu Yuanfei, Lévy Laurence, Neuveut Christine, Prieur Xavier, Flajolet Marc, Prigent Sylvie, Buendia Marie-Annick (February 2003). "Identification of the LIM protein FHL2 as a coactivator of beta-catenin". J. Biol. Chem. (United States) 278 (7): 5188–94. doi:10.1074/jbc.M207216200. ISSN 0021-9258. PMID 12466281.
^ Lange, Stephan; Auerbach Daniel, McLoughlin Patricia, Perriard Evelyne, Schäfer Beat W, Perriard Jean-Claude, Ehler Elisabeth (December 2002). "Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2". J. Cell. Sci. (England) 115 (Pt 24): 4925–36. doi:10.1242/jcs.00181. ISSN 0021-9533. PMID 12432079.
^ McLoughlin, Patricia; Ehler Elisabeth, Carlile Graeme, Licht Jonathan D, Schäfer Beat W (October 2002). "The LIM-only protein DRAL/FHL2 interacts with and is a corepressor for the promyelocytic leukemia zinc finger protein". J. Biol. Chem. (United States) 277 (40): 37045–53. doi:10.1074/jbc.M203336200. ISSN 0021-9258. PMID 12145280.
^ ^a ^b ^c ^d ^e Wixler, V; Geerts D, Laplantine E, Westhoff D, Smyth N, Aumailley M, Sonnenberg A, Paulsson M (October 2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes". J. Biol. Chem. (UNITED STATES) 275 (43): 33669–78. doi:10.1074/jbc.M002519200. ISSN 0021-9258. PMID 10906324.
^ Müller, J M; Isele U, Metzger E, Rempel A, Moser M, Pscherer A, Breyer T, Holubarsch C, Buettner R, Schüle R (February 2000). "FHL2, a novel tissue-specific coactivator of the androgen receptor". EMBO J. (ENGLAND) 19 (3): 359–69. doi:10.1093/emboj/19.3.359. ISSN 0261-4189. PMC 305573. PMID 10654935.
^ Samson, Thomas; Smyth Neil, Janetzky Stefanie, Wendler Olaf, Müller Judith M, Schüle Roland, von der Mark Helga, von der Mark Klaus, Wixler Viktor (July 2004). "The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor". J. Biol. Chem. (United States) 279 (27): 28641–52. doi:10.1074/jbc.M312894200. ISSN 0021-9258. PMID 15117962.
^ ^a ^b Fimia, G M; De Cesare D, Sassone-Corsi P (November 2000). "A Family of LIM-Only Transcriptional Coactivators: Tissue-Specific Expression and Selective Activation of CREB and CREM". Mol. Cell. Biol. (UNITED STATES) 20 (22): 8613–22. doi:10.1128/MCB.20.22.8613-8622.2000. ISSN 0270-7306. PMC 102166. PMID 11046156.
^ Purcell, Nicole H; Darwis Dina, Bueno Orlando F, Müller Judith M, Schüle Roland, Molkentin Jeffery D (February 2004). "Extracellular Signal-Regulated Kinase 2 Interacts with and Is Negatively Regulated by the LIM-Only Protein FHL2 in Cardiomyocytes". Mol. Cell. Biol. (United States) 24 (3): 1081–95. doi:10.1128/MCB.24.3.1081-1095.2004. ISSN 0270-7306. PMC 321437. PMID 14729955.
^ Ng, Enders Kai On; Chan Kwok Keung, Wong Chi Hang, Tsui Stephen Kwok Wing, Ngai Sai Ming, Lee Simon Ming Yuen, Kotaka Masayo, Lee Cheuk Yu, Waye Mary Miu Yee, Fung Kwok Pui (2002). "Interaction of the heart-specific LIM domain protein, FHL2, with DNA-binding nuclear protein, hNP220". J. Cell. Biochem. (United States) 84 (3): 556–66. doi:10.1002/jcb.10041. ISSN 0730-2312. PMID 11813260.
^ Yan, Jinghua; Zhu Jianhua, Zhong Hongjun, Lu Qiujun, Huang Cuifen, Ye Qinong (October 2003). "BRCA1 interacts with FHL2 and enhances FHL2 transactivation function". FEBS Lett. (Netherlands) 553 (1–2): 183–9. doi:10.1016/S0014-5793(03)00978-5. ISSN 0014-5793. PMID 14550570.
^ Yan, Jing-Hua; Ye Qi-Nong, Zhu Jian-Hua, Zhong Hong-Jun, Zheng Hui-Yong, Huang Cui-Fen (December 2003). "[Isolation and characterization of a BRCA1-interacting protein]". Yi Chuan Xue Bao (China) 30 (12): 1161–6. ISSN 0379-4172. PMID 14986435.
^ Li, H Y; Ng E K, Lee S M, Kotaka M, Tsui S K, Lee C Y, Fung K P, Waye M M (2001). "Protein-protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET)". J. Cell. Biochem. (United States) 80 (3): 293–303. doi:10.1002/1097-4644(20010301)80:3<293::AID-JCB10>3.0.CO;2-U. ISSN 0730-2312. PMID 11135358.
^ Amaar, Yousef G; Thompson Garrett R, Linkhart Thomas A, Chen Shin-Tai, Baylink David J, Mohan Subburaman (April 2002). "Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2)". J. Biol. Chem. (United States) 277 (14): 12053–60. doi:10.1074/jbc.M110872200. ISSN 0021-9258. PMID 11821401.
^ Bai, Shuting; Zha Jikun, Zhao Haibo, Ross F Patrick, Teitelbaum Steven L (November 2008). "Tumor Necrosis Factor Receptor-associated Factor 6 Is an Intranuclear Transcriptional Coactivator in Osteoclasts". J. Biol. Chem. (United States) 283 (45): 30861–7. doi:10.1074/jbc.M802525200. ISSN 0021-9258. PMC 2662164. PMID 18768464.
^ Tanahashi, H; Tabira T (September 2000). "Alzheimer's disease-associated presenilin 2 interacts with DRAL, an LIM-domain protein". Hum. Mol. Genet. (ENGLAND) 9 (15): 2281–9. doi:10.1093/oxfordjournals.hmg.a018919. ISSN 0964-6906. PMID 11001931.

[edit] Further reading

Genini M, Schwalbe P, Scholl FA, et al. (1997). "Subtractive cloning and characterization of DRAL, a novel LIM-domain protein down-regulated in rhabdomyosarcoma". DNA Cell Biol. 16 (4): 433–42. doi:10.1089/dna.1997.16.433. PMID 9150430.
Chan KK, Tsui SK, Lee SM, et al. (1998). "Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart". Gene 210 (2): 345–50. doi:10.1016/S0378-1119(97)00644-6. PMID 9573400.
Chan KK, Tsui SK, Ngai SM, et al. (2000). "Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47". J. Cell. Biochem. 76 (3): 499–508. doi:10.1002/(SICI)1097-4644(20000301)76:3<499::AID-JCB16>3.0.CO;2-4. PMID 10649446.
Müller JM, Isele U, Metzger E, et al. (2000). "FHL2, a novel tissue-specific coactivator of the androgen receptor". EMBO J. 19 (3): 359–69. doi:10.1093/emboj/19.3.359. PMC 305573. PMID 10654935.
Wixler V, Geerts D, Laplantine E, et al. (2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes". J. Biol. Chem. 275 (43): 33669–78. doi:10.1074/jbc.M002519200. PMID 10906324.
Tanahashi H, Tabira T (2001). "Alzheimer's disease-associated presenilin 2 interacts with DRAL, an LIM-domain protein". Hum. Mol. Genet. 9 (15): 2281–9. doi:10.1093/oxfordjournals.hmg.a018919. PMID 11001931.
Fimia GM, De Cesare D, Sassone-Corsi P (2000). "A Family of LIM-Only Transcriptional Coactivators: Tissue-Specific Expression and Selective Activation of CREB and CREM". Mol. Cell. Biol. 20 (22): 8613–22. doi:10.1128/MCB.20.22.8613-8622.2000. PMC 102166. PMID 11046156.
Scholl FA, McLoughlin P, Ehler E, et al. (2000). "Dral Is a P53-Responsive Gene Whose Four and a Half Lim Domain Protein Product Induces Apoptosis". J. Cell Biol. 151 (3): 495–506. doi:10.1083/jcb.151.3.495. PMC 2185594. PMID 11062252.
Li HY, Kotaka M, Kostin S, et al. (2001). "Translocation of a human focal adhesion LIM-only protein, FHL2, during myofibrillogenesis and identification of LIM2 as the principal determinants of FHL2 focal adhesion localization". Cell Motil. Cytoskeleton 48 (1): 11–23. doi:10.1002/1097-0169(200101)48:1<11::AID-CM2>3.0.CO;2-I. PMID 11124707.
Li HY, Ng EK, Lee SM, et al. (2001). "Protein-protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET)". J. Cell. Biochem. 80 (3): 293–303. doi:10.1002/1097-4644(20010301)80:3<293::AID-JCB10>3.0.CO;2-U. PMID 11135358.
Dye BT, Patton JG (2001). "An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles". Exp. Cell Res. 263 (1): 131–44. doi:10.1006/excr.2000.5097. PMID 11161712.
Ng EK, Chan KK, Wong CH, et al. (2002). "Interaction of the heart-specific LIM domain protein, FHL2, with DNA-binding nuclear protein, hNP220". J. Cell. Biochem. 84 (3): 556–66. doi:10.1002/jcb.10041. PMID 11813260.
Amaar YG, Thompson GR, Linkhart TA, et al. (2002). "Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2)". J. Biol. Chem. 277 (14): 12053–60. doi:10.1074/jbc.M110872200. PMID 11821401.
Stilo R, Leonardi A, Formisano L, et al. (2002). "TUCAN/CARDINAL and DRAL participate in a common pathway for modulation of NF-kappaB activation". FEBS Lett. 521 (1–3): 165–9. doi:10.1016/S0014-5793(02)02869-7. PMID 12067710.
McLoughlin P, Ehler E, Carlile G, et al. (2002). "The LIM-only protein DRAL/FHL2 interacts with and is a corepressor for the promyelocytic leukemia zinc finger protein". J. Biol. Chem. 277 (40): 37045–53. doi:10.1074/jbc.M203336200. PMID 12145280.
Jiang LQ, Wen SJ, Wang HY, Chen LY (2003). "Screening the proteins that interact with calpain in a human heart cDNA library using a yeast two-hybrid system". Hypertens. Res. 25 (4): 647–52. doi:10.1291/hypres.25.647. PMID 12358155.
Schlisio S, Halperin T, Vidal M, Nevins JR (2002). "Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function". EMBO J. 21 (21): 5775–86. doi:10.1093/emboj/cdf577. PMC 131074. PMID 12411495.
Lange S, Auerbach D, McLoughlin P, et al. (2003). "Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2". J. Cell. Sci. 115 (Pt 24): 4925–36. doi:10.1242/jcs.00181. PMID 12432079.
Wei Y, Renard CA, Labalette C, et al. (2003). "Identification of the LIM protein FHL2 as a coactivator of beta-catenin". J. Biol. Chem. 278 (7): 5188–94. doi:10.1074/jbc.M207216200. PMID 12466281.

[edit] External links

FHL2 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

This article on a gene on chromosome 2 is a stub. You can help Wikipedia by expanding it.

[pmid8753811-1] Morgan MJ, Madgwick AJ (October 1996). "Slim defines a novel family of LIM-proteins expressed in skeletal muscle". Biochem. Biophys. Res. Commun. 225 (2): 632–8. doi:10.1006/bbrc.1996.1222. PMID 8753811.

[entrez-2] "Entrez Gene: FHL2 four and a half LIM domains 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=2274.

[pmid12466281-3] Wei, Yu; Renard Claire-Angélique, Labalette Charlotte, Wu Yuanfei, Lévy Laurence, Neuveut Christine, Prieur Xavier, Flajolet Marc, Prigent Sylvie, Buendia Marie-Annick (February 2003). "Identification of the LIM protein FHL2 as a coactivator of beta-catenin". J. Biol. Chem. (United States) 278 (7): 5188–94. doi:10.1074/jbc.M207216200. ISSN 0021-9258. PMID 12466281.

[pmid12432079-4] Lange, Stephan; Auerbach Daniel, McLoughlin Patricia, Perriard Evelyne, Schäfer Beat W, Perriard Jean-Claude, Ehler Elisabeth (December 2002). "Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2". J. Cell. Sci. (England) 115 (Pt 24): 4925–36. doi:10.1242/jcs.00181. ISSN 0021-9533. PMID 12432079.

[pmid12145280-5] McLoughlin, Patricia; Ehler Elisabeth, Carlile Graeme, Licht Jonathan D, Schäfer Beat W (October 2002). "The LIM-only protein DRAL/FHL2 interacts with and is a corepressor for the promyelocytic leukemia zinc finger protein". J. Biol. Chem. (United States) 277 (40): 37045–53. doi:10.1074/jbc.M203336200. ISSN 0021-9258. PMID 12145280.

[pmid10906324-6] Wixler, V; Geerts D, Laplantine E, Westhoff D, Smyth N, Aumailley M, Sonnenberg A, Paulsson M (October 2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes". J. Biol. Chem. (UNITED STATES) 275 (43): 33669–78. doi:10.1074/jbc.M002519200. ISSN 0021-9258. PMID 10906324.

[pmid10654935-7] Müller, J M; Isele U, Metzger E, Rempel A, Moser M, Pscherer A, Breyer T, Holubarsch C, Buettner R, Schüle R (February 2000). "FHL2, a novel tissue-specific coactivator of the androgen receptor". EMBO J. (ENGLAND) 19 (3): 359–69. doi:10.1093/emboj/19.3.359. ISSN 0261-4189. PMC 305573. PMID 10654935.

[pmid15117962-8] Samson, Thomas; Smyth Neil, Janetzky Stefanie, Wendler Olaf, Müller Judith M, Schüle Roland, von der Mark Helga, von der Mark Klaus, Wixler Viktor (July 2004). "The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor". J. Biol. Chem. (United States) 279 (27): 28641–52. doi:10.1074/jbc.M312894200. ISSN 0021-9258. PMID 15117962.

[pmid11046156-9] Fimia, G M; De Cesare D, Sassone-Corsi P (November 2000). "A Family of LIM-Only Transcriptional Coactivators: Tissue-Specific Expression and Selective Activation of CREB and CREM". Mol. Cell. Biol. (UNITED STATES) 20 (22): 8613–22. doi:10.1128/MCB.20.22.8613-8622.2000. ISSN 0270-7306. PMC 102166. PMID 11046156.

[pmid14729955-10] Purcell, Nicole H; Darwis Dina, Bueno Orlando F, Müller Judith M, Schüle Roland, Molkentin Jeffery D (February 2004). "Extracellular Signal-Regulated Kinase 2 Interacts with and Is Negatively Regulated by the LIM-Only Protein FHL2 in Cardiomyocytes". Mol. Cell. Biol. (United States) 24 (3): 1081–95. doi:10.1128/MCB.24.3.1081-1095.2004. ISSN 0270-7306. PMC 321437. PMID 14729955.

[pmid11813260-11] Ng, Enders Kai On; Chan Kwok Keung, Wong Chi Hang, Tsui Stephen Kwok Wing, Ngai Sai Ming, Lee Simon Ming Yuen, Kotaka Masayo, Lee Cheuk Yu, Waye Mary Miu Yee, Fung Kwok Pui (2002). "Interaction of the heart-specific LIM domain protein, FHL2, with DNA-binding nuclear protein, hNP220". J. Cell. Biochem. (United States) 84 (3): 556–66. doi:10.1002/jcb.10041. ISSN 0730-2312. PMID 11813260.

[pmid14550570-12] Yan, Jinghua; Zhu Jianhua, Zhong Hongjun, Lu Qiujun, Huang Cuifen, Ye Qinong (October 2003). "BRCA1 interacts with FHL2 and enhances FHL2 transactivation function". FEBS Lett. (Netherlands) 553 (1–2): 183–9. doi:10.1016/S0014-5793(03)00978-5. ISSN 0014-5793. PMID 14550570.

[pmid14986435-13] Yan, Jing-Hua; Ye Qi-Nong, Zhu Jian-Hua, Zhong Hong-Jun, Zheng Hui-Yong, Huang Cui-Fen (December 2003). "[Isolation and characterization of a BRCA1-interacting protein]". Yi Chuan Xue Bao (China) 30 (12): 1161–6. ISSN 0379-4172. PMID 14986435.

[pmid11135358-14] Li, H Y; Ng E K, Lee S M, Kotaka M, Tsui S K, Lee C Y, Fung K P, Waye M M (2001). "Protein-protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET)". J. Cell. Biochem. (United States) 80 (3): 293–303. doi:10.1002/1097-4644(20010301)80:3<293::AID-JCB10>3.0.CO;2-U. ISSN 0730-2312. PMID 11135358.

[pmid11821401-15] Amaar, Yousef G; Thompson Garrett R, Linkhart Thomas A, Chen Shin-Tai, Baylink David J, Mohan Subburaman (April 2002). "Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2)". J. Biol. Chem. (United States) 277 (14): 12053–60. doi:10.1074/jbc.M110872200. ISSN 0021-9258. PMID 11821401.

[pmid18768464-16] Bai, Shuting; Zha Jikun, Zhao Haibo, Ross F Patrick, Teitelbaum Steven L (November 2008). "Tumor Necrosis Factor Receptor-associated Factor 6 Is an Intranuclear Transcriptional Coactivator in Osteoclasts". J. Biol. Chem. (United States) 283 (45): 30861–7. doi:10.1074/jbc.M802525200. ISSN 0021-9258. PMC 2662164. PMID 18768464.

[pmid11001931-17] Tanahashi, H; Tabira T (September 2000). "Alzheimer's disease-associated presenilin 2 interacts with DRAL, an LIM-domain protein". Hum. Mol. Genet. (ENGLAND) 9 (15): 2281–9. doi:10.1093/oxfordjournals.hmg.a018919. ISSN 0964-6906. PMID 11001931.

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FHL2

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