Ecdysone 20-monooxygenase

ecdysone 20-monooxygenase
Identifiers
EC number	1.14.99.22
CAS number	55071-97-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, an ecdysone 20-monooxygenase (EC 1.14.99.22) is an enzyme that catalyzes the chemical reaction

ecdysone + AH₂ + O₂ $\rightleftharpoons$ 20-hydroxyecdysone + A + H₂O

The 3 substrates of this enzyme are ecdysone, an electron acceptor AH₂, and O₂, whereas its 3 products are 20-hydroxyecdysone, the reduction product A, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derive from O miscellaneous. The systematic name of this enzyme class is Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating). Other names in common use include alpha-ecdysone C-20 hydroxylase, and ecdysone 20-hydroxylase.

References

• Johnson P, Rees HH (1977). "The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria". Biochem. J. 168 (3): 513–20. PMC 1183799. PMID 606249. 
• Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE (1976). "Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)". Experientia. 32 (4): 438–9. doi:10.1007/BF01920781. PMID 5286. 
• Gilbert LI; Bollenbacher, WE; Cooper, DY; Schleyer, H; Wielgus, JJ; Gilbert, LI (1979). "Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase". Mol. Cell. Endocrinol. 15 (3): 111–33. doi:10.1016/0303-7207(79)90033-9. PMID 488526.