PIPES
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This article is about the biochemical buffer PIPES. For other uses, see Pipe (disambiguation).
| PIPES | |
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1,4-Piperazinediethanesulfonic acid (IUPAC)
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Other names
PIPES
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| Identifiers | |
| CAS number | 5625-37-6 |
| Properties | |
| Molecular formula | C8H18N2O6S2 |
| Molar mass | 302.37 |
| Appearance | White powder |
| Melting point |
Decomposes above 300 °C |
| Boiling point |
Decomposes |
| Solubility in water | 1 g/L (100 °C) |
| Hazards | |
| MSDS | External MSDS |
| Main hazards | Irritant |
| NFPA 704 |
 0
1
0
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 (what is this?) (verify)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references |
PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]
[edit] Applications
PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.
[edit] References
- ^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
- ^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
- ^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
- ^ Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry 33 (2): 110–8. PMID 3918095. http://www.jhc.org/cgi/content/abstract/33/2/110.
[edit] See also
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