28 Days has flashbacks of the main character and her sister trying to wake their mother when they find her collapsed in the living room subverted, as she was just passed out from alcohol, and just when they're getting worried a sharp slap brings her around. However, when she really dies, the girls assume it's the same thing, and suggest "slapping her REAL hard" to their aunt, who's come to tell them.
Inverted in 28 Days Later, in which the suicide note left by the hero's parents bids him not to wake up from his coma to face the Zombie Apocalypse.
Virgil Brigman: God damn it, you bitch! You never backed away from anything in your life! Now FIGHT!
Amadeus: The title character's wife Constanze does this to Mozart after his body finally gives out after slaving away for months on his final composition, a Requiem mass ironically enough.
At the end of The Amazing Spider-Man 2, after the fight with Green Goblin, when Spidey thinks he saved Gwen Stacy. He starts happily, then softly, then, when he realises something's wrong, imploringly, then he begs and finally breaks down completely. Made even more tearjerker-ish by the fact that viewers without keen eyes might think that Gwen actually made it...note It would seem that Spidey managed to catch her in time, but for a moment you can see that the back of her head actually hit the ground the moment he caught her...
In The Astronaut Farmer, the family's youngest goes to get her grandpa from the living room for dinner. She comes back, saying "Grandpa won't wake up."
Neytiri tries desperately to wake Jake up. He's not in that body at the time. And again at the end, but this time she figures out what's up and what she needs to do to help him.
A mishearing provides an accidental example: When Eytukan is killed, Neytiri says something that sounds very much like, "Wakey, wakey," which spoils the mood entirely.
Avengers: Endgame has a strange case where it's played straight by one character (Spider-Man/Peter Parker) and inverted by another (Pepper). Iron Man delivers the Badass Fingersnap that puts an end to Thanos and his army for good, and the raw power of this action, in a homebrew gauntlet, is far too much for a human to handle. Once it's done and the villains are gone, Tony collapses. Peter, his protege for the entire Infinity Saga, swings over and tries to get him to wake up, but his wife, Pepper, realizes what he's done and tells him he can rest now as he breathes his last.
Babe: Pig in the City: When Fugly Floom is dragged to the hospital, where he later dies, Thelonius says that he tried and failed to wake him up.
This version shows the enchanted objects (temporarily) turning completely inanimate. It's already hard to watch, but to twist in the knife even further, just after Cadenza and Gaderobe become inanimate, their dog-turned-footstool, Frou-Frou, runs over and barks at them and paws at them, desperately trying to get a response out of either one of them, clearly not understanding what's happened... before Frou-Frou becomes inanimate, too. It's heartbreaking. (But, on the upside, the curse is broken a few minutes later and the now-human Cadenza and Gaderobe being reunited with their dog is adorable.)
Around the same point in the film, after the Beast (temporarily) dies, Belle is reduced to weeping "Come back!" It's the film's tearjerker page's trope image for a reason.
Armand at the end of Camille (1936), when he realizes Marguerite has died in his arms: "No, don't leave me! Marguerite, come back!"
Possibly the most soulcrushing and heartrending example in live-action film is the 1979 remake of The Champ, wherein the titular character, (Jon Voight) dies in the locker room shortly after winning a brutal boxing match and his young son, played by Ricky Schroeder (whose acting chops here bury most full-grown adult actors and netted a Golden Globe), goes into an emotional meltdown trying to get his dad to wake up. You can watch it here. It's so sad that at leastonestudy on the biology of crying used it as a reliable way to produce tears.
Dancing Trees: After Josephina is shot, her daughter Martha runs out of the car and starts tapping her arm, saying, "Mom, get up. Ice cream. Ice cream, one scoop. Just one scoop." A variation in that Josephina isn't quite dead yet, although she will be soon.
When Peg asks Edward Scissorhands what happened to his father, he replies, "He didn't wake up."
Occurs in The Fall, when Alexandria tries to wake up Roy, after he falls asleep, having taken "sleeping pills" that we later find out are placebos.
The Fantastic Four: First Steps: After Sue dies overexerting her powers to defeat Galactus, Reed desperately does CPR, telling her to stay with him. She only comes back to life when Franklin uses his powers for the first time.
Occurs in the film Godzilla vs. Destoroyah when Junior dies. Godzilla tries desperately to wake him up, and when he realizes he's lost his son, he cries.
Halloween (2007): Laurie finds Lynda's body in Michael Myers' lair and desperately tries to wake her before realizing she is dead.
Liz has one of these moments with the red guy himself in Hellboy II: The Golden Army, including citing various reasons for him to wake up. The one that works: "You're going to be a father..." At that point, he murmurs, "I... become... father?" and forces himself back alive. Liz also has a moment in the comics, when Abe dies; it's not an explicit version of this trope, but the way she shakes his body and keeps calling his name, even after other characters have informed her of his death, implies she was still trying to wake him up.
Bilbo: No! No, no, no, no, no, no! Thorin! No, don't you dare... Thorin... Thorin, Thorin, hold on... Hold on, please... The Eagles... The Eagles... The Eagles are here... Thorin! (breaks down in childlike, whimpering tears)
In Independence Day, when the President's wife, her mother dies, then-8-year-old Mae Whitman's character asks her Dad "Is she sleeping now?"
Jane: Helen. Are you awake? It's morning. Helen! [screams]
Jason's Lyric: When his girlfriend, Lyric, is (accidentally) shot by his own brother, Joshua, Jason tearfully pleads her to stay strong by reminding about their plan to escape town together (after he initially called it off, out of brotherly love). Thankfully, Lyric miraculously survives as she and Jason are seen riding a bus together in the end.
Jason: Lyric... Lyric... I'm ready to go... Come on, you should get up... You gotta get up 'cause we gotta go. Come on.
Genuinely tragic moment in The Joy Luck Club when An Mei's mother commits her carefully timed suicide and An Mei does this. Yi Ding was a terrific actress.
In the extended version of the film, Éomer rushing screaming to Éowyn's side and shaking her has all of the techniques. It's actually quite effective; Éomer is usually The Stoic, seeing him screaming wordlessly in grief is downright shaking.
In The Maze Runner (2014), Thomas asks Chuck to stay with him, begging him to wake up, and refuses to leave his body behind as it sinks in that he's dead after Taking the Bullet for Thomas.
Jon Voight does this as Joe Buck in Midnight Cowboy, when his only friend (and arguable love interest) Rizzo dies on the bus in the end. (Voight would later be on the receiving end of this trope in The Champ.)
OK Rico? Rico? Rico? Hey, Rico? Rico?
In The Mummy Returns, Rick pleads his wife Evy to "come back!" and crashes into Heroic BSoD. And thanks to the Book of the Dead, it's only temporary anyway. She gets better.
Subverted in National Treasure: Book of Secrets: in the prologue, after the Lincoln assassination conspirator kills his father, young Thomas Gates kneels by the body and begs him to "come back," but then immediately afterward cries out that it is unfair.
James Bond: It's all right. It's quite all right, really. She's having a rest. We'll be going on soon. There's no hurry, you see. We have all the time in the world.
In Pihu, the eponymous two-year-old repeatedly tries to wake her dead mother.
At the end of The Room (2003), Mark attempts to wake up Johnny... after the latter shot himself through the mouth.
Non-verbally done in Return of the Jedi, where the AT-STs are firing at the Ewoks, and hit a pair dead on... and one of them gets up and starts nudging the other.
In Serenity (2005), Zoe hadsa mental snap and refuses to believe that Wash is dead, even though it is very clear. This is less about the tragic misunderstanding of death and more about how Wash was Zoe's emotional anchor. She does revert to her military training by the time she and Mal leave the ship, however, and by the end...
"She's tore up plenty. But she'll fly true."
SHAZAM! Fury of the Gods has Shazam makes a Heroic Sacrifice to defeat Kalypso and Ladon by invoking the lightning that strikes him during his transformations to essentially nuke them, but of course, also turns him back into Billy, an ordinary human boy who can't withstand such an attack. After the battle is over, his foster brother and best friend Freddy finds his body in the wreckage, covered with electrical burns, and at first eagerly encourages him to wake up by assuring him he won, before growing more and more frantic and tearful as he realizes Billy really is dead, finally shouting "You asshole!" and breaking down in sobs. Luckily for everyone, Billy's brought Back from the Dead by Diana.
In SLC Punk!, Stevo goes downstairs only to find his best friend, Heroin Bob, dead. In a truly heartbreaking scene, he dissolves into a sobbing mess and screams at Heroin Bob.
Stevo: "ONLY POSERS DIE, YOU FUCKING IDIOT! Now what am I gonna do for a friend? You're my only friend! Please wake up now! Please, I'm sorry!"
A prolonged version of this in Steel Magnolias when M'Lynn Eatonton is trying to rouse Shelby when she's in the hospital after her final diabetic episode. Not long after, she's taken off life support.
Terror at Black Falls: After Quito's death, Juan holds him in his arms, saying, "Wake up, my Quito, listen to me, wake up, Quito!" Carlos finally tells Juan that he is dead.
Averted in Threads. In post-nuclear war Britain, society has degenerated to medieval levels and those born after the war are mentally stunted and barely able to speak English. Jane only says "Ruth... work... work... up," and shows little emotion once she realises her mother is dead.
Titanic (1997): Rose, excited about a lifeboat coming back, tries to wake Jack up... for a full minute... and then breaks down to the point where the boat passed her by...
The Winning of Barbara Worth: No dialogue to this effect but toddler Barbara can be seen trying to shake her dead mother awake as Worth and his party arrive at the spot in the desert where Barbara's mother collapsed.
. It's so sad that at least