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. 2016 Aug;54(7):1153-8.
doi: 10.1093/chromsci/bmw095.

Analysis of 25 C NBOMe in Seized Blotters by HPTLC and GC-MS

Boris Duffau  1 , Cristian Camargo  2 , Marcelo Kogan  2 , Edwar Fuentes  2 , Bruce Kennedy Cassels  3
Affiliations

Affiliations

  • 1 Drug Analysis Section, Public Health Institute of Chile, Santiago, Chile bduffau@gmail.com bduffau@ispch.cl.
  • 2 Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile.
  • 3 Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile.

Analysis of 25 C NBOMe in Seized Blotters by HPTLC and GC-MS

Boris Duffau et al. J Chromatogr Sci. 2016 Aug.
. 2016 Aug;54(7):1153-8.
doi: 10.1093/chromsci/bmw095.

Authors

Boris Duffau  1 , Cristian Camargo  2 , Marcelo Kogan  2 , Edwar Fuentes  2 , Bruce Kennedy Cassels  3

Affiliations

  • 1 Drug Analysis Section, Public Health Institute of Chile, Santiago, Chile bduffau@gmail.com bduffau@ispch.cl.
  • 2 Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile.
  • 3 Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile.

Abstract

Use of unauthorized synthetic drugs is a serious, forensic, regulatory and public health issue. In this scenario, consumption of drug-impregnated blotters is very frequent. For decades, blotters have been generally impregnated with the potent hallucinogen known as lysergic acid diethylamide (LSD); however, since 2013 blotter stamps with N-2 methoxybenzyl-substituted phenylethylamine hallucinogen designated as "NBOMes" have been seized in Chile. To address this issue with readily accessible laboratory equipment, we have developed and validated a new HPTLC method for the identification and quantitation of 25-C-NBOMe in seized blotters and its confirmation by GC-MS. The proposed method was validated according to SWGTOX recommendations and is suitable for routine analysis of seized blotters containing 25-C-NBOMe. With the validated method, we analyzed 15 real samples, in all cases finding 25-C-NBOMe in a wide dosage range (701.0-1943.5 µg per blotter). In this situation, we can assume that NBOMes are replacing LSD as the main hallucinogenic drug consumed in blotters in Chile.

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Figures

Figure 1.

Figure 1.

Structure of 25-C-NBOMe.

Figure 1.

Structure of 25-C-NBOMe.

Figure 1.
Structure of 25-C-NBOMe.
Figure 2.

Figure 2.

TIC and mass spectrum of…

Figure 2.

TIC and mass spectrum of standard of 25-C-NBOMe. This figure is available in…

Figure 2.
TIC and mass spectrum of standard of 25-C-NBOMe. This figure is available in black and white in print and in color at JCS online.
Figure 3.

Figure 3.

Results of purity and spectrum…

Figure 3.

Results of purity and spectrum comparison of real samples and reference material of…

Figure 3.
Results of purity and spectrum comparison of real samples and reference material of 25-C-NBOMe. This figure is available in black and white in print and in color at JCS online.
Figure 4.

Figure 4.

Densitogram with 25-C-NBOMe, LSD and…

Figure 4.

Densitogram with 25-C-NBOMe, LSD and DMT as an example of selectivity and separation…

Figure 4.
Densitogram with 25-C-NBOMe, LSD and DMT as an example of selectivity and separation capability of the proposed method. This figure is available in black and white in print and in color at JCS online.
Figure 5.

Figure 5.

The calibration plot.

Figure 5.

The calibration plot.

Figure 5.
The calibration plot.
See this image and copyright information in PMC

References

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