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. 2011 Apr 18;50(17):3878-81.
doi: 10.1002/anie.201008178. Epub 2011 Mar 23.

Genetically encoded copper-free click chemistry

Tilman Plass  1 , Sigrid Milles, Christine Koehler, Carsten Schultz, Edward A Lemke
Affiliations

Affiliation

  • 1 Structural and Computational Biology Unit, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Free PMC article

Genetically encoded copper-free click chemistry

Tilman Plass et al. Angew Chem Int Ed Engl. .
Free PMC article
. 2011 Apr 18;50(17):3878-81.
doi: 10.1002/anie.201008178. Epub 2011 Mar 23.

Authors

Tilman Plass  1 , Sigrid Milles, Christine Koehler, Carsten Schultz, Edward A Lemke

Affiliation

  • 1 Structural and Computational Biology Unit, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
No abstract available

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Figures

Scheme 1

Scheme 1

a) Structures of UAAs 1 …

Scheme 1

a) Structures of UAAs 1 and 2 , and fluorogenic dye 3 .…

Scheme 1
a) Structures of UAAs 1 and 2, and fluorogenic dye 3. b) Reaction scheme for copper-free click reaction with fluorogenic azides.
Figure 1

Figure 1

a) Fluorescent images of E.…

Figure 1

a) Fluorescent images of E. coli suspensions in microcentrifuge tubes expressing GFP TAG …

Figure 1
a) Fluorescent images of E. coli suspensions in microcentrifuge tubes expressing GFPTAG in the absence (−) and presence (+) of 1 and 2. b) Corresponding Coomassie stained SDS PAGE gel after purification of GFPTAG by a C-terminal affinity handle.
Figure 2

Figure 2

a) 2D histogram (S versus …

Figure 2

a) 2D histogram (S versus E FRET ) of smFRET data of GFP …

Figure 2
a) 2D histogram (S versus EFRET) of smFRET data of GFPTAG→1,Atto647N molecules. b) Cells incubated with 3 expressing tRNApyl/pylRSAF with mCherryTAG→1,3 (plus differential interference contrast (DIC) image) and mCherryWT, imaged for fluorescence of coumarin and mCherry. c) Emission scan for coumarin of E. coli suspensions used in (b), mCherryTAG→1,3=red, mCherryWT=black. Scale bar=5 μm.
See this image and copyright information in PMC

References

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