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. 1999 Apr 30;450(1-2):77-83.
doi: 10.1016/s0014-5793(99)00446-9.

Differential association of cytoplasmic signalling molecules SHP-1, SHP-2, SHIP and phospholipase C-gamma1 with PECAM-1/CD31

N J Pumphrey  1 , V Taylor, S Freeman, M R Douglas, P F Bradfield, S P Young, J M Lord, M J Wakelam, I N Bird, M Salmon, C D Buckley
Affiliations

Affiliation

  • 1 Division of Immunity and Infection, University of Birmingham, UK.

Differential association of cytoplasmic signalling molecules SHP-1, SHP-2, SHIP and phospholipase C-gamma1 with PECAM-1/CD31

N J Pumphrey et al. FEBS Lett. .
. 1999 Apr 30;450(1-2):77-83.
doi: 10.1016/s0014-5793(99)00446-9.

Authors

N J Pumphrey  1 , V Taylor, S Freeman, M R Douglas, P F Bradfield, S P Young, J M Lord, M J Wakelam, I N Bird, M Salmon, C D Buckley

Affiliation

  • 1 Division of Immunity and Infection, University of Birmingham, UK.

Abstract

Recent studies have shown that, in addition to its role as an adhesion receptor, platelet endothelial cell adhesion molecule 1/CD31 becomes phosphorylated on tyrosine residues Y663 and Y686 and associates with protein tyrosine phosphatases SHP-1 and SHP-2. In this study, we screened for additional proteins which associate with phosphorylated platelet endothelial cell adhesion molecule 1, using surface plasmon resonance. We found that, besides SHP-1 and SHP-2, platelet endothelial cell adhesion molecule 1 binds the cytoplasmic signalling proteins SHIP and PLC-gamma1 via their Src homology 2 domains. Using two phosphopeptides, NSDVQpY663TEVQV and DTETVpY686SEVRK, we demonstrate differential binding of SHP-1, SHP-2, SHIP and PLC-gamma1. All four cytoplasmic signalling proteins directly associate with cellular platelet endothelial cell adhesion molecule 1, immunoprecipitated from pervanadate-stimulated THP-1 cells. These results suggest that overlapping immunoreceptor tyrosine-based inhibition motif/immunoreceptor tyrosine-based activation motif-like motifs within platelet endothelial cell adhesion molecule 1 mediate differential interactions between the Src homology 2 containing signalling proteins SHP-1, SHP-2, SHIP and PLC-gamma1.

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