Papers by Ratna Meidyawati
Majalah Kedokteran Bandung, 2020
Escherichia coli (E.coli) is a frequently found infectious pathogen commonly transmitted through ... more Escherichia coli (E.coli) is a frequently found infectious pathogen commonly transmitted through water. In Indonesia, the level of this pathogen exceeds the accepted standard. Several studies have shown the presence of antibiotic-resistant E.coli, making studies on alternative treatments for E.coli necessary. Abrus precatorius L. and Piper betle L. leaves are among herbs that have herbal antibacterial properties. This study observed and compared the antibacterial effects of Abrus precatorius L. and Piper betle L. leaves against E.coli. This was an in vitro experimental study performed at the
International Journal of Applied Pharmaceutics, 2017
Objectives: The purpose of this study is to evaluate the influence of grape seed extract (GSE) so... more Objectives: The purpose of this study is to evaluate the influence of grape seed extract (GSE) solution as dentin biomodification on microleakagebetween composite resin and dentin.Methods: A total of 52 dentin specimens were divided into two groups. Group 1 involved the application of GSE solution for 30 seconds after etching.Group 2 served as the control group.Results: The GSE solution group significantly increases microleakage between the composite resin and dentin compared to the control group.Conclusions: The application of GSE solution after etching can increase microleakage between composite resin and dentin.
Journal of International Dental and Medical Research, 2019
Sodium hypochlorite (NaOCl) as a root canal irrigant during endodontic treatment, may dissolve or... more Sodium hypochlorite (NaOCl) as a root canal irrigant during endodontic treatment, may dissolve organic part of the dentin structure, especially collagen. The aim of this study was to analyze the effect of irrigation with 2.5% and 5.25% NaOCl on collagen in dentin and shear bond strength (SBS) values of total-etch adhesive system to dentin with nanofilled composite resin NaOCl. Fifteen human maxillary premolars were halved, which produced 30 specimens that were randomly divided into 3 groups of 10 each for Mallory Azan staining. For the SBS test, 10 different maxillary premolar were sliced into 3 parts each and were divided into 3 groups of 10 each. In each test, 2 of the groups were irrigated with 2.5% and 5.25% NaOCl, and one unirrigated group was used as control. Mallory Azan staining was performed to evaluate the presence of collagen. Evaluation of the specimens irrigated by different concentrations of NaOCl and control group revealed that increasing NaOCl concentration reduced t...
Journal of International Dental and Medical Research, 2017
Despite the excellence properties of mineral trioxide aggregate sealer (MTAS), it may have some i... more Despite the excellence properties of mineral trioxide aggregate sealer (MTAS), it may have some influence in its sealing ability. The purpose of this study was to analyze and compare sealing ability of MTAS and resin epoxy sealer (RES). Thirty-two extracted human lower premolar teeth had their canals prepared with ProTaper Universal. This group divided into two groups, group 1, the root canals were filled using guttaperca with MTAS and Group 2 root canals were filled using guttaperca with RES. All samples were incubated at the temperature of 370C for 24 hours with 100% humidity. Then the whole sample were immersed in India ink for 7 days. Samples were washed and decalcified until became transparent. The penetration of India ink was evaluated by using a stereo microscope, and the scores are were given by criteria: score 1: 0-0.5 mm ink penetration; a score 2: ink penetration 0.511mm; and a score 3: ink penetration > 1 mm. Score 1 in the group RES 37.5%, and 21.9% MTAS group, score...
Journal of International Dental and Medical Research, 2019
Enterococcus faecalis is the common bacteria causing persistent root canal infections, and its vi... more Enterococcus faecalis is the common bacteria causing persistent root canal infections, and its virulence is 1000 times stronger in biofilms than planktons. Chlorhexidin is a golden standard irrigation material in eliminating E. faecalis in endodontic treatment, however it has cytotoxicity, which can increase the number of free radicals with risk of cell death. Due to some side effect of chemical irrigation solutions used during root canal preparation, it is useful to seek for herbal alternatives with better profile of side effects and equivalent efficacy. Thus, this study aimed to analyze the efficacy of secang heartwood extract in eliminating E. faecalis biofilm. The study sample was the patient with a diagnosis of chronic apical periodontitis and chronic apical abscess. Bacterial growth of E. faecalis clinical isolates bacteria was seen from 4 patients (total 7 patients) and confirmed through bacterial DNA with conventional PCR and electrophoresis tests. Biofilms were exposed to v...
Polymer-induced liquid-precursor (PILP) process used polyaspartic acid as an analog noncollagenou... more Polymer-induced liquid-precursor (PILP) process used polyaspartic acid as an analog noncollagenous protein to prevent spontaneous nucleation of calcium and phosphate before entering the intrafibrillar compartment. Fluoride was added for the formation of fluorapatite crystals. Objective of this study is determining the effect on dentinal hardness when fluoride is added in the PILP process. Twenty-four dentin blocks were divided into four groups. Group I (normal dentin) was the control, Group II comprised demineralized dentin stored in PILP remineralization solution, Group III comprised demineralized dentin stored in remineralized PILP solution with 5 ppm fluoride, Group IV comprised demineralized dentin stored in remineralized PILP solution with 25 ppm fluoride. All samples were stored in an incubator with continuous shaking at 37 °C for 14 days. A Vickers hardness test was performed to measure dentin hardness along with an X-ray diffraction (XRD) test to determine the percentage of ...
Journal of International Dental and Medical Research, 2019
E. faecalis bacteria are frequently encountered in persistent and resistant root canal infections... more E. faecalis bacteria are frequently encountered in persistent and resistant root canal infections. Their ability to form biofilms contributes to its persistence. Importantly, chemical-based irrigation usage is linked to side effects. Caesalpinia Sappan Linn, known as Secang heartwood, has been traditionally used for a variety of medical purposes, including antimicrobial. We aimed analyze the antibacterial effects of Secang heartwood extract solution on E. faecalis biofilms. E. faecalis biofilms from clinical isolates were grown on microtiter well plates, incubated for 24 h and subjected to various concentrations of Secang heartwood extract solution: 312 μg/ml, 625 μg/ml, 1250 μg/ml, 2500 μg/ml, 5000 μg/ml (and CHX 2% as control). Following a 15 min incubation, wells were rinsed with PBS and scrapped off. Biofilms were diluted, spread on a solid medium and incubated for 24 h. Subsequently, viable grown colonies were counted. The concentration of Secang heartwood extract solution that...
This study aimed to evaluate the effects of epoxy resin and calcium silicate-based root canal sea... more This study aimed to evaluate the effects of epoxy resin and calcium silicate-based root canal sealer on fiber post adhesion strength. Thirty single-rooted lower premolars were divided into three groups (n = 10 for each group): Group 1, no root canal filling; Group 2, epoxy resin root canal sealer; and Group 3, calcium silicate root canal sealer. After all teeth were prepared with rotary instruments, Groups 2 and 3 were filled with gutta-percha points and root canal sealer. A fiber post was cemented in all tooth, then 2-mm disc samples were taken from the middle root section and subjected to a push-out bond strength test. Data were analyzed using one-way ANOVA and Bonferroni test. The highest average adhesion strength was exhibited by the control group (57.142 ± 12.205 MPa), followed by the epoxy resin group (44.455 ± 10.347 MPa) and then the calcium silicate group (28.009 ± 9.562 MPa). There was a significant difference in push-out bond strength between the groups (p < 0.006). Th...
Journal of International Dental and Medical Research, 2018
Enterococcus faecalis bacteria are root canal pathogens that can migrate to periodontal tissue in... more Enterococcus faecalis bacteria are root canal pathogens that can migrate to periodontal tissue in true combined endo-perio lesions through apical foramen, dentinal tubules, and lateral and accessory canals. The same strain of E. faecalis in root canals and pocket periodonsium tissue in true combined endo-perio lesions showed pathological unity and the possibility of migration. The quantity of E. faecalis contributes to the disease’s severity because of its virulence, i.e., substance aggregation, lipoteichoic acid, extracellular superoxide, gelatinase, hyaluronidase, and hemolysin. To analyze the strain type and quantity of the E. faecalis taken from root canals and pockets of true combined endo-perio lesions, primary endodontic lesions, and primary periodontal lesions. 16 samples were taken from the following three diagnosis groups: true combined endo-perio lesions (root canal and pocket), primary endodontic lesions, and primary periodontal lesions. The strain types were analyzed vi...
Journal of International Dental and Medical Research, 2017
To analyze the antibacterial effect of Bioceramic and mineral trioxide aggregate (MTA) sealers ag... more To analyze the antibacterial effect of Bioceramic and mineral trioxide aggregate (MTA) sealers against Enterococcus faecalis clinical isolates at 2 minutes (fresh), 4 hours (initial setting), 1 day, and 7 days after the sealers were mixed. The antibacterial effects of Bioceramic and MTA sealers were assessed by direct contact tests. Each sealer directly contacted clinical isolates of E. faecalis at 2 minutes, 4 hours, 1 day, and 7 days after the sealers were mixed. The suspension was swabbed in an agar medium and incubated for 24 hours to determine the number of bacterial colonies that grew. The colonies in the agar plates were counted with colony-forming units (CFUs). There were significant differences between Bioceramic and MTA at 7 days, between Bioceramic at 2 minutes (fresh) and 4 hours (initial setting), and between Bioceramic at 4 hours (initial setting) and 7 days. Both Bioceramic and MTA sealers showed good antibacteria l effects against E. faecalisat fresh, initial setting...
International Journal of Applied Pharmaceutics, 2018
Objective: The elimination of bacteria from the root canal has always been a problem in root cana... more Objective: The elimination of bacteria from the root canal has always been a problem in root canal management, and Enterococcus faecalis often found in the persistent intraradicular infections that occur after poor or unsuccessful endodontic treatments. The use of an irrigant with a diode laser adjunct eliminates this bacteria. To analyze and compare the effectiveness of a diode laser, chlorhexidine 2%, and natrium hypochlorite 2.5% on a clinical isolate of E. faecalis biofilms.Methods: Using E. faecalis biofilms from clinical isolate were grown on microtiter well plate, incubated for 24 h and subjected to the following treatments: Natrium hypochlorite 2.5% (5 s), chlorhexidine 2% (5 s), natrium chloride 0.9% (5 s), and the irrigants with additional diode laser irradiation (980 nm, 15 Hz, 1.5 W, 3.5 J, 5 s). The antibacterial effects of the irrigants and diode laser were scored using colony form units (CFU).Results: The clinical isolate colony of E. faecalis that was exposed to a sa...
International Journal of Applied Pharmaceutics, 2018
Objective: The aim of this study was to evaluate the antibacterial potential of NAC as an endodon... more Objective: The aim of this study was to evaluate the antibacterial potential of NAC as an endodontic irrigant on the clinical isolates of the Enterococcus faecalis biofilm.Methods: NAC with a pH of 2.5 and 11 (NAC pH 2.5 and NAC pH 11, respectively) were exposed to clinical isolates of E. faecalis biofilms for 1 min. The NAC samples were compared to 2% chlorhexidine (CHX), which is commonly used as an irrigant in persistent infections. The antibacterial potential of these irrigants was evaluated by comparing the bacterial count of the E. faecalis colonies after they were exposed to the irrigants.Results: The NAC pH 2.5 test group showed a reduction in the E. faecalis colonies, but this reduction was not statistically significant when compared to the 2% CHX group results. The NAC pH 11 test group showed the greatest reduction in bacterial colonies, and this reduction was statistically significant when compared to the NAC pH 2.5 and 2% CHX groups’ results.Conclusion: NAC pH 11 has ant...
International Journal of Applied Pharmaceutics, 2020
Objective: Root canal preparation procedures can produce a smear layer when in contact with the r... more Objective: Root canal preparation procedures can produce a smear layer when in contact with the root canal wall, which can result in treatmentfailure. As such, the cross-section shape of the file may influence the production of smear layer. In this study, we compared the smear layer productionat the apical third of the root canal wall between files with a triangular or rectangular cross-section shape.Methods: Thirty-two human premolar samples taken from mandibles were divided into two groups whose root canals were prepared using fileswith either a triangular (One Curve®, n=16) or rectangular (Hyflex EDM®, n=16) cross-section shape. After preparation, the root canals were irrigatedwith a combination of 2.5% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA). The smear layers in the apical third of the root canal walls wereobserved using a scanning electron microscope and quantified according to the Foschi scoring system.Results: The Mann–Whitney U-test revealed a significant differ...
International Journal of Applied Pharmaceutics, 2020
Objective: The main objective of endodontic treatment is to remove microorganisms from the root c... more Objective: The main objective of endodontic treatment is to remove microorganisms from the root canal space and prevents reinfection. Deep penetration of the dentinal tubule is advantageous because it increases the contact area between the root canal filling material and dentin, thereby increasing the sealing quality of the entire root canal system. Bioceramic sealers are biocompatible, nontoxic, non-shrinking, hydrophilic, and stable, do not expand during setting, and can form hydroxyapatite, which then forms chemical bonds with dentin to compare the abilities of three types of bioceramic-based sealers to penetrate the dentinal tubules.Methods: Obturation used three types of bioceramic sealers. Group 1 (calcium phosphate silicate), Group 2 (a mixture of tricalcium silicate and resin), and Group 3 (pure tricalcium silicate) were observed using a scanning electron microscope and measurement of the penetration distance with ImageJ.Results: The bioceramic sealers had statistically sign...
International Journal of Applied Pharmaceutics, 2020
Objective: Endodontic infections are treated with a root canal; one of the stages involves using ... more Objective: Endodontic infections are treated with a root canal; one of the stages involves using an irrigation solution to eliminate microorganisms.Sodium hypochlorite (NaOCl) is an irrigation solution used to eliminate Fusobacterium nucleatum, the common cause of primary endodonticinfections. However, this material is synthetic and can cause side effects and inflammation of the periapical tissues. Thus, an irrigation solution, suchas xanthorrhizol, which is made of natural ingredients, is required. However, xanthorrhizol’s antibacterial effect against F. nucleatum has never beenassessed. Thus, this study aimed to analyze the effect of xanthorrhizol, derived from Curcuma xanthorrhiza Roxb., against F. nucleatum ATCC 25586biofilm.Methods: The methylthiazol tetrazolium (MTT) assay test and colony count test were performed to assess F. nucleatum eradication after exposure toxanthorrhizol at various concentrations (0.5%, 0.75%, 1%, 1.25%, and 1.5%) and 2.5% NaOCl, which was used as posi...
International Journal of Applied Pharmaceutics, 2020
Objective: The objective of this study was to analyze the intrafibrillar remineralization process... more Objective: The objective of this study was to analyze the intrafibrillar remineralization process and the size of hydroxyapatite crystals formed.Methods: The samples were divided into four groups. The control group was immersed in a demineralization solution, whereas the remaining threetreatment groups were immersed in a remineralization solution containing polyaspartic acid for 3, 7, or 14 days. The effect of polyaspartic acid onintrafibrillar remineralization during the polymer-induced liquid-precursor (PILP) process was evaluated using transmission electron microscopy,and the size of hydroxyapatite crystals was examined through X-ray diffraction.Results: Significant differences were detected in the intrafibrillar remineralization between the treatment groups (subjected to remineralization for3, 7, and 14 days) and the demineralized dentin control group. However, no significant differences were detected in the size of hydroxyapatite crystalsbetween the control and the treatment gr...
International Journal of Applied Pharmaceutics, 2020
Objective: Caries treatment can be performed by minimal intervention, i.e., by removing the infec... more Objective: Caries treatment can be performed by minimal intervention, i.e., by removing the infected dentin and leaving the affected dentin and theninducing remineralization in the affected dentin. The affected dentin still consists of collagen cross bonds. Polymer-induced liquid precursor processis a guided tissue remineralization method that aims to remineralize intrafibrillar and extrafibrillar dentin by adding polymers that are similar tonon-collagen protein. One of the non-collagen protein analog materials is polyaspartic acid. The aim of this study was to evaluate the remineralizationof dentin on the demineralized dentin surface after immersed in remineralization solutions containing polyaspartic acid as a non-collagen proteinanalog.Methods: Human premolar teeth extracted for orthodontic purposes were divided into four groups, namely, one control and three treatment groups.Teeth in the control group were immersed only in the demineralization solution containing acetate buffer ...
Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2018
Objective: To compare lateral compaction obturation with carrier-based gutta-percha and downpack-... more Objective: To compare lateral compaction obturation with carrier-based gutta-percha and downpack-backfill. Material and Methods: Ninety tooth with single root canal were prepared with rotary Protaper and divided into 3 groups: Group 1 obturated with lateral heated compaction (LHC), Group 2 with carrier-based gutta-percha (CP) and Group 3 with downpack-backfill (DB). The apical one-third adaptation was determined by examining the dye penetration between obturation material and root canal wall on the horizontal cut samples. The data received was analyzed using SPSS 17 software. Chi-square statistical test was done with level of significance (α) of 0.05. Results: The DB group had the highest amount of score of 0, followed by CP group and LHC group. The DB group had 28 samples (93.3%) with score of 0, which was the largest compared to the CP and LHC group. All groups had some score 2, and score 3 and 4 were only examined in the LHC group Adaptation of the apical one-third on DB group had the best result, followed by CP and LHC group (p>0.05). Conclusion: The adaptation of apical one-third by downpack-backfill was the best among the three groups, but there was no statistically significant difference among those groups.
International Journal of Applied Pharmaceutics, 2019
Objective: The objective of this study was to compare the potential mutagenicity of resin-, silic... more Objective: The objective of this study was to compare the potential mutagenicity of resin-, silicone-, and bioceramic-based sealers on proteinexpression in human lymphocytes. There has been limited research on resin-, silicone-, and bioceramic-based sealers effects on protein expressionin lymphocytes.Methods: Nine samples of each sealer were incubated in 2 mL human blood for 1, 3, and 7 days. Then, the isolated lymphocytes are observed forprotein separation by electrophoresis method. Profile of protein bands observed and data were analyzed statistically by Kruskal–Wallis and post hocMann–Whitney.Results: Although no statistically significant differences in protein bands were observed among the resin-, silicone-, and bioceramic-based sealers(p=0.111), there was a statistically significant difference between the resin- and silicone-based sealers on the 1st day (p=0.046) and 3rd day (p=0.046)and between the silicone- and bioceramic-based sealers on the 1st day (p=0.046). Thus, the pres...
International Journal of Applied Pharmaceutics, 2019
Objective: Candida albicans is a predominant fungal species found in persistent root canal infect... more Objective: Candida albicans is a predominant fungal species found in persistent root canal infection, and its virulence depends on the ability to formbiofilms. To be able to eliminate this infection, a safe irrigation solution during root canal preparation is needed. This study is conducted to investigatethe antifungal properties of secang heartwood extract solutions on C. albicans biofilm.Methods: C. albicans biofilm was obtained from incubation of C. albicans ATCC 10231 at 96-well plates in 37°C for 24 h. These biofilms were exposedto various treatments: Secang heartwood in three different concentrations (25%, 33%, and 50%), sodium hypochlorite (NaOCl) 2.5%, and controlgroup. The results of the treatment are to see the optical density (OD) value. The higher the OD values, the lower antifungal activity of the solution.Results: The mean OD result from six samples obtained the mean value of various OD values showed in ELISA reader. Antifungal efficacy of 25%secang heartwood was lower...
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Papers by Ratna Meidyawati