A new 5′ terminal murine GAPDH exon identified using 5′RACE LaNe
@article{Park2005AN5,
title={A new 5′ terminal murine GAPDH exon identified using 5′RACE LaNe},
author={Daniel J. Park},
journal={Molecular Biotechnology},
year={2005},
volume={29},
pages={39-46},
url={https://api.semanticscholar.org/CorpusID:45702164}
}A ligation-independent, fully gene-specific, nested polymerase chain reaction (PCR) method for the elucidation of 5′ cDNA sequence is described and demonstrated for the first time and a new GAPDH 5′ exon was discovered using 5′RACE LaNe.
6 Citations
Lariat-dependent nested PCR for flanking sequence determination.
- 2011
Biology, Chemistry
Methods detailed in this chapter relate to the use of Lariat-dependent Nested (LaNe) PCR to characterize unknown RNA or DNA sequence flanking known regions. A multitude of approaches designed to…
RESIDUAL DISEASE IN CHRONIC MYELOID LEUKEMIA AFTER INDUCTION OF MOLECULAR REMISSION
- 2011
Medicine
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to…
Complete genomic sequence of an infectious pancreatic necrosis virus isolated from rainbow trout (Oncorhynchus mykiss) in China
- 2016
Biology, Environmental Science
The sequence of ChRtm213 was the first gene sequence of IPNV isolates in China, and the results suggest that the Chinese IPNV isolate has relative closer relationship with Japanese IPNV strains.
17 References
3' RACE LaNe: a simple and rapid fully nested PCR method to determine 3'-terminal cDNA sequence.
- 2004
Biology
3′ RACE LaNe is demonstrated: a simple and rapid fully nested PCR method to determine 3′-terminal cDNA sequence that outperforms a conventional heminested anchored PCR 3′ Race series when it is applied to the long and complex tammar wallaby ATRX gene and the mouse PGK1 and GAPD genes.
CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs.
- 1999
Biology, Chemistry
The method combines the 5'-CAP-dependent addition of specifically three to four non-templated dCMP residues to the 3'-end of full-length cDNAs by reverse transcriptases in the presence of manganese and the controlled ribonucleotide tailing of cDNA ends by terminal deoxynucleotidyl transferase using rATP.
Ligation-mediated amplification of RNA from murine erythroid cells reveals a novel class of beta globin mRNA with an extended 5'- untranslated region
- 1994
Biology
The evolutionary conservation of this relationship suggests the importance of the GATA promoter element of the mouse beta globin gene and its possible involvement in developmental regulation of expression of this gene.
A general method for cloning eukaryotic structural gene sequences.
- 1976
Biology
The susceptibility of inserted beta globin, sequences to the restriction endonuclease EcoRI confirms the existence of a site already found through previous nucleotide sequence analysis, as well as estimating the amount of inserted globin sequences.
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
- 1988
Biology
The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.
Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.
- 1976
Biology
Data indicate that a loop structure on the 3' end of cDNA allowed DNA synthesis to take place by a "self-priming" mechanism, and some of the double-stranded DNA synthesized corresponded to the entire sequence of the 9S mRNA template.
A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.
- 1988
Biology
A procedure that extends this technique to sequences that lie outside the boundaries of known sequences by inversion of the sequence of interest by circularizaton and re-opening at a different site is described.